This is a table of TCR transgenic mice and the corresponding models of negative selection compiled from the literature.
If any errors or ommisions are discovered, please contact Troy Baldwin at baldw026@umn.edu with the correction.

Model of Negative selection

Name

First described

MHC Class

TCR transgenes

Positve selection background

Antigen

(1) Endogenous Peptide

(2)
Transgenic Peptide

(3)
SuperAntigen

(4)
Allogeneic MHC

Stage of deletion

References

Sha & Loh et al., Nature 1988 336:73

Va3/Vb8

H-2^b

L^d alloantigen (syn. peptide SIYRYYGL H-2K^b)

"SIY" peptide K14promoter

L^d

1) Sykulev & Eisen, PNAS 1994 91:11487 and Ukada & Eisen, PNAS 1993 90:11272 identified endogenous peptides from a-KGDH.
2) Follow-up to MHC restriction in Russell & Loh, JI 1990 144:3318.
3) SIY transgene reported by McGargill & Hogquist, 2002 JI 169:2141 and Mayerova & Hogquist JI 2004 172:851.
4) Identification of SIY peptide by Udaka & Walden et al., JI 1996 157:670.

BM3.3

Auphan & Schmitt-Verhulst et al., 1994 24:1572

?/?

H-2^k

H-2K^b/PBM1 alloantigen

Kb Transgenics

1) Deletion reported in original article.
2) Alloreactive peptide identified by Guimezanes & Schmitt-Verhulst et al., EJI 2001 31:421.

Morahan & Miller et al., PNAS 1991 88:11421

Va8/Vb11

H-2^bm1

K^b alloantigen

RIP-K^b

H-2K^b expressing strains

1) Morahan & Miller et al., PNAS 1991 88:11421.

Mamalaki & Kioussis et al., Dev Imm 1993 3:159

Va4/Vb11

H-2^b (D^b)

influenza nucleoprotein 366-374

NP transgenic

I-E⁺/Mtv8/9/11

3) SP

1) Deletion reported in original article.

HY (B6.2.12)

Kisielow & von Boehmer et al., Nature 1988 333:742

Va17/Vb8.2

H-2^b (D^b)

mouse male antigen SMCY 738-764

Male mice

K14-male antigen transgenic

1) DP

1) Kisielow & von Boehmer, Nature 1988 335:730 shows MHC restriction of HY.
2) HY transgene reported by Mayerova & Hogquist, JI 2004 172:851.

HY^cd4

Baldwin & Hogquist et al., JEM 2005 202:111

Va17/Vb8.2

H-2^b (D^b)

mouse male antigen SMCY 738-764

Male mice

1) SP

Kb5.C20, Des

Schonrich & Arnold et al., Cell 1991 65:293

?/?

H-2^k, H-2^kxd

H-2K^b alloantigen

H-2K^b expressing strains

4) DP

1) Transgene H-2K^b and crossing to H-2K^b reported in original article.
2) Deletion studied further by Simon & Schmitt-Verhulst et al., Cell Death & Differentiation 2000 7:1253.
3) Identification of peptides reported in Guimezanes & Schmitt-Verhulst et al., EJI 2001 31:421.

N15

Ghendler & Reinherz et al., EJI 1997 27:2279.

Va8/Vb5.2

H-2^b (K^b)

Vesicular stomatitis virus 52-59

1) Caspase block of negative selection reported by Clayton & Reinherz et al., Embo 1997 16:2282.
2) FG loop mutation blocks negative selection reported in Sasada & Reinherz et al., JEM 2002 195:1419.
3) In vivo peptide injection reported in original article describing N15.

NOD.AI4, AI4

Graser & Serreze et al., JI 2000 164:3913

Va8/Vb2

H-2^g7 (NOD) (K^d)

Uncharacterized beta cell antigen

B6.H2^g7

4) DP

1) Deletion reported in Choisy-Rossi & Serreze et al., JI 2004 173:3791.

OT-1

Hogquist & Carbone et al., Cell 1994 76:17

Va2/Vb5

H-2^b (K^b)

Chicken Ovalbumin 257-264

RIP-OVA, K14-OVAp K14-OVA, Act-OVA

I-E⁺/Mtv 6/9⁺ strains

2) SP

1) Receptor editing phenomenon described in McGargill & Hogquist, NI 2000 1:336 and Mayerova & Hogquist, JI 2004 172:851.
2) BM OT-I into RIP-mOVA reported by Kurts & Heath et al., JEM 1997 186:239.
3) bCat peptide found by Santori & Jameson, Imm 2002 17:131.
4) Mtv suceptibility Hogquist, personal comm.
5) Deletion by RIP-mOVA transgene reported by Gallegos & Bevan, JEM 2004 200:1039.
6) Deletion in RIP-OVA mice also reported in Leishman & Cheroutre et al., Imm 2002 16:355.

P14

Pircher & Zinkernagel et al., Nature 1989 342:559.

Va2/Vb8.1

H-2^b (D^b)

LCMV GP33

neonatal (chronic) LCMV infection

Mls^a

2) DP
3) SP

1) Mlsa and LCMV deletion are reportedin Pircher & Zinkernagel, et al., Nature 1989 342:559.

P1ACTL

Sarma & Liu et al., JEM 1999 189:811

Va8/Vb1

H-2^d (L^d)

P1A 35-43

P1A (no deletion)

Emmb-P1A

1) deletion with Tg P1A described in orginal article

QM11

Suzuki & Shinohara et al., JI 1994 153:4496

Va10/Vb4

H-2^q

I-A^kalloreactive

strains expressing I-A^k

4) DP

1) Deletion reported in original article.

ST40

Hoffman and Schild et al., EJI 2004 34:1798

Va16/Vb1

H-2^b (D^b)

Adenovirus E1a 234-243

1) No deletion reported in original article.

ST42

Hoffman and Schild et al., EJI 2004 34:1798

Va16/Vb1

H-2^b (D^b)

Adenovirus E1a 234-243

1) No deletion reported in original article.

RT1

Yokosuka & Saito et al., JEM 2002 195:991

Va42/Vb8.1

H-2^d (D^d)

HIV-1 gp160

Doucey & Leuscher et al., JBC 2003 278:3257

Va?/Vb?

H-2^b

P. berghei circumsporozoite Ag

TG-B

Geiger & Flavell PNAS 1992 89:2985

Va5/Vb8

H-2^k (K^k)

SV40 Large T Antigen

RIP-SV40

1) SV40-T transgenes reported in original paper and Geiger & Flavell, JI 1993 151:7030.

6C5

Sullivan & Jensen et al., Imm 2002 17:95.

Va3.2/Vb5.1

H-2^b or ^d (Qa-1^b)

human insulin B chain

1) Positve selection doesnt require Qdm reported by Sullivan & Jensen et al., JI 2004 173:6065.

C10.4 TCRtrans+

Berg & Staerz et al., Imm 1999 11:33.

Va4.2/Vb8.1

H-2^b (H-2M3)

Listeria monocytogenes AttM f-MFFINILTL

2) Positive selection endogenous ligand (partial agonist) described by Berg & Staerz et al., JI 2000 165:4209.

Chiu & Wang et al., JEM 1999 190:1869

Va10.2/Vb5.2

H-2^b or ^d (H2-M3)

Listeria monocytogenes LemA protein

1) Chiu & Wang et al., JEM 1999 190:1869

1H3.1

Viret & janeway JI 2000 164:4627

Va1/Vb6

H-2^b (I-A^b)

peptide from I-Ea chain

I-A^b/Ea peptide

1) Negative selection reported in original article.
2) Positive selection to the Ea-MHC fusion protein reported by Viret & Janeway et al., PNAS 2001 98:9243.

2B4

Berg & Davis et al., Cell 1989 58:1035

Va?/Vb3

H-2^a (I-E^k)

Pigeon cytochrome C (PCC) 81-104

PCC transgenic

Mls-2a and Mls-3a

1) Deletion reported in original article.

3.L2

Kersh & Allen et al., JI 1998 161:585

Va18/Vb8.3

H-2^k (I-E^k)

human hemoglobin d allele 64-76

Hbb d Tg, tet HEL/Hbd Tg

2) DP

1) Deletion reported in original article.
2) Deletion from solubleHEL/Hbd reported in Haribhai & Williams et al., 2003 170:3007.

3A9

Ho & Davis et al., JEM 1994 179:1539

Va3/Vb8.2

H-2^k (I-A^k)

hen egg lysozyme (HEL) 46-61

HEL Tg, tet HEL/Hbd Tg

2) DP

1) Deletion reported by Haribhai & Williams et al., JI 2003 170:3007.

4.1, NY4.1

Schmidt & Santamaria et al., JEM 1997 186:1059

Va4.1/Vb11

H-2^g7 (I-A^g7)

uncharacterized beta cell antigen

NOD beta cell Antigen

1) SP

1) Deletion reported in original article.
2) Follow-up reportedin Thiessen & Santamaria et al., Diabetes 2002 51:325

4B2A1

Bogen & Dembic et al., EJI 1992 22:703

Va1, Vb8.2

H-2^d (I-E^d)

MOPC315 IgG2 91-101

MOPC315 BCR Tg

2) DP

1) Deletion in g2315 Tg animals reported by Bogen & Weiss et al., Embo 1993 12:357.

5C.C7

Seder & de St. Groth et al., JEM 1992 176:1091.

Va11, Vb3

H-2^a (I-E^k)

PCC/MCC 88-104

MX-MCC-Hel Tg

1) Block of positive selection by p/MHC specific Ab G35 reported by Baldwin & Davis et al., JEM 1999 189:13.
2) MCC-5C.C7 double transgenic reported by Girgis & de St. Groth et al., JEM 1999 189:265.

6.5, HA, TS1

Kirberg & von Boehmer et al., JEM 1994 180:25

Va4/Vb8.2

H-2^d (I-E^d)

Influenza hemaglutinnin (HA) 111-119

Igk-HA, RIP-HA, SV40-HA, Pev-HA, b-myo HA

1) IgK driven HA Transgene reported in Lanoue & Sarukhan JEM 1997 185:405.
2)RIP drive HA transgene reported in Sarukhan & von Boehmer Imm 1998 8:563.
3) Other HA promoters tested by Jordan & Caton et al., NI 2001 2:301 and Reed & Naji et al., JI 2003 171:6502.

A18, C5

Zal & Stockinger et al., JEM 1994 180:2089

Va11.1/Vb8.3

H-2^a (I-E^k)(C5a negative)

complement protein C5 107-121

CBA mouse strain (C5a+)

1) SP

1) Negative selection in C5+ mice reported in original paper.

A23

McHugh & Natarajan et al., EJI 2001 31:2094

Va2/Vb2

H-2^d (I-A^d)

H/K ATPase a-chain 633-641

1) no deletion reported in original article.

ABLE

Reiner & Locksley et al., JI 1998 160:884.

Va8.2/Vb4

H-2^d (I-A^d)

Leishmania major LACK

ABM or 3BBM74

Backstrom & Palmer et al., Sci 1998 281:835

Va2/Vb8

H-2^b

I-A^bm12

B6.bm12

4) SP

1) Deletion reported in original article.

AND

Kaye & Hedrick et al., Nature 1989 341:746

Va11/Vb3

I-E^k or I-A^b (I-E^kor I-A^b)

MCC 88-103/PCC 88-104

K^b-PCC transgenic, I-E^k MCC peptide transgenic

I-A^s

1) Vasquez & Hedrick, JEM 1992 175:1307 show I-A^s negatively selects.

Granucci & Ricciardi-Castagnoli et al., JEM 1996 183:203

Va4/Vb14

H-2^d (I-A^d)

IgG2ab 435-451

1) No deletion reported by original article.

BDC2.5

Katz & Mathis et al., Cell 1993 74:1089

Va1/Vb4

H-2^g7 NOD

Uncharacterized islet cell antigen

NOD, no overt deletion seen

D011.10

Murphy & Loh et al., Science 1990 250:1720

Va?/Vb8.2

H-2^d (I-A^d)

chicken ovalbumin 323-339

Ld-nOVA transgenic

I-Ab

1) Negative selection is induced by peptide injection in original paper.
2) Negative selection can be mediated by several types of APC in vitro reported by Iwabuchi & Loh et al., PNAS 1992 89:9000.

D10, D10.G4.1

Sant'Angelo & Janeway et al., Imm 1996 4:367

Va2/Vb8.2

H-2^k (I-A^k)

chicken conalbumin 134-146

H-2 b, q, d, f, r, and u

4) H-2b,q or d DP
H-2f,r or u SP

1) Deletion reported by Sant'Angelo & Janeway et al., PNAS 2002 99:6931.

Dep

Klein & Kyewski et al., JEM 1998 188:5

Va11.2/Vb5.1

H-2^b (I-A^b)

human Reactive C Protein 89-101

hCRP Tg

2) DP

1) Deletion reported in original article.

Ea6

Viret & Janeway et al., JI 2000 165:6183

Va23.1/Vb6

H-2^b (I-A^b)

I-Ea-chain 52-66

I-Ead transgenic

G2, 2.102

Hsu & Allen et al., JEM 1995 181:805

endogenous a/Vb1

H-2^k (I-E^k)

mouse hemoglobin d allele 64-76

Hbbd⁺ strains

1) Deletion reported in original article.

G286, B16.3, (G206)

Tarbell & McDevitt et al., JEM 2002 196:481

Va4.5/Vb1

mouse glutamic acid decarboxylase 65 286-300

NOD

1) DP

1) Deletion also reported in McDevitt Ann NY Acad 2003 1005:75.

He & Bottomly et al., Nat Imm 2002 3:127

Va10/Vb8

(I-A^s)

human collagen IV a2 chain 675-686

KRN, R28

Kouskoff & Mathis et al., Cell 1996 87:811

Va4/Vb6

H-2^k (I-A^k)

bovine pancreas RNAse Alloreactive to H-2^g7 41-61

Ea-Glucose 6-phosphate isomerase peptide transgenic

NOD

4) SP (more evident in mice < 2wks old)

1) Deletion reported in original article.

MBP, 121-150

Huseby & Goverman et al., Imm 2001 14:471

Va2/Vb8

H-2^u (I-A^u)

murine myelin basic protein 121-150

endogenous MBP

1) SP

1) Deletion reported in original article.

MBP, 172.1

Goverman & Zaller et al., Cell 1993 72:551

Va2.3/Vb8.2

H-2^u (I-A^u)

murine Myelin Basic Protein 1-11

1) No deletion reported in original article.

MBP, 19G

Lafaille & Tonegawa et al., Cell 1994 78:399

Va?/Vb8

H-2^u (I-A^u)

murine Myelin Basic Protein 1-11

1) No deletion reported in original article.

OT-II

Barnden & Carbone et al., Immunol Cell Bio 1998 76:34.

Va2/Vb5

H-2^b (I-A^b)

chicken ovalbumin protein 323-339

RIP-OVA

2) SP

1) Deletion by RIP-mOVA transgene reported by Gallegos & Bevan, JEM 2004 200:1039.
2) Positive selection reported in Wong & Rudensky et al., JI 200 164:6252.

Sep

Klein & Kyewski et al., JEM 1998 188:5

Va4/Vb8.3

H-2^b (I-A^b)

human Reactive C Protein 80-94

hCRP Tg

2) SP

1) Deletion reported in original article.

SM1

McSorley & Jenkins et al., Imm 2002 16:365

Va10/Vb2

H-2^b (I-A^b)

salmonella flagellin 427-441

Smarta

Oxenius & Hengartner et al., EJI 1998 28:390

Va2.3/Vb8.3

H-2^b (I-A^b)

LCMV GP 61-80

neonatal LCMV infection

2) DP

1) Oxenius & Hengartner et al., EJI 1998 28:390.

TCli

Wong & Rudensky et al., JI 2000 164:6252

Va18/Vb6

H-2^b (I-A^b)

human invariant chain CLIP fragment81-104

1) Positive selection reported in Wong & Rudensky et al., JI 200 164:6252.

TCR-HNT

Scott & Lo et al., Imm 1994 1:73

Va?/Vb8

H-2^d (I-A^d)

influenza virus hemaglutinin 126-138

1) No deletion reported in original article.

TEa

Grubin & Rudensky et al., Imm 1997 7:197

Va2/Vb6

H-2^b (I-A^b)

murine I-E^a-chain 52-68

strains expressing I-Ea

1) partial deletion SP

1) Positive & negative selection reported in original article.
2) Positive selection reported in Wong & Rudensky et al., JI 200 164:6252.

TS1(SW)

Jordan & Caton et al., Nat. Imm 2001 4:301

Va8.3/Vb10

H-2^d (I-E^d)

SW influenza hemagglutinin 111-119

1) no deletion reported in original article

DN (CD1)

Cheng & Strober et al., JI 1996 156:3591

Va4.4/Vb9

H-2^d

Probable alloreactivity to CD1

1) Further characterized in Hueber & Otz, Arthritis & Rheumatism 2004 50:3239

SP (CD1)

Cheng & Strober et al., JI 1996 156:3591

Va4.4/Vb9

H-2^d

Probable alloreactivity to CD1

1) Further characterized in Hueber & Otz, Arthritis & Rheumatism 2004 50:3239