Author: Unknown Date: Unknown

Magnetic Columns Selection

1. Harvest cells; count
2. Stain w/ Bio IgG
I’ve been staining in HBSS 1% FCS, 0.5ul of 1mg/mL Bio IgG per 5X10^6 cells in 100 ul of HBSS 1% FCS
3. Wash 1x in labeling buffer
PBS 495mL
0.5 M EDTA 2mL
BSA 2.5 mL
2% Azide 2.5 mL
3. Resuspend in 1.5 mL labeling buffer
4. Add appropriate amount of SA-beads (vortex beads well before dispensing), and incubate on ice for 15 minutes
1ul per 10^6 cells is recommended amount
5. Prime column during this incubation w/ 500ul labeling buffer--be sure not to introduce any bubbles onto the column
6. Wash 1x in labeling buffer, and then resuspend cells in labeling buffer at 500ul /100x10^6 cells and apply to column through filter
7. Collect fraction and 4 0.5 mL washes.
8. Elute column with 1 mL labeling buffer and syringe.

Column Info

Mini column
Total cells: 2 x 10^8
Target cells: up to 10^7

Midi column
Total cells: 2 x 10^9
Target cells: up to 10^8