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Protocols

These protocols are ones used by the Hogquist/Jameson lab. They should all have an author and a date at the top. Old protocols have probably been changed in the lab, but changes might not be entered on the web page. If you are attempting to use these protocols it would be best to e-mail the author to see if anything has changed.

For a detailed discusion of fetal thymic organ culture (FTOC) protocol please read:

Assays of thymic selection. Fetal thymus organ culture and in vitro thymocyte dulling assay.2001. K. A. Hogquist. Methods Mol Biol 156: 219-32

For Hogquist/Jameson lab members: use the following template when writing new protocols. Send the finished protocol to the lab safety officer (currently is Jie Ding) and the webmaster (currently is Matthew Wilken) so they can put them on the computer and the webpage.

Standard Operating Procedure Template

Adenovirus
Agarose Gels
Antibody production
BM chimeras
BrdU labelling
cDNA prep
Cell Milking
CFSE labelling
Con A Stimulation
Cr51 Release Assay
CTL Stimulator-Feeders
Chromatin Immunoprecipitation (ChiP)
Contact Hyper Sensitivity

DNA extraction
DNA transfection
DP dulling
FACS analysis
FACS staining-blood
FITC conjugation (Cellite)
Fixing in paraformaldehyde

FTOC
Fusion Protein Protocol
Genomic DNA Prep
Intrathymic Injections

Intracellular Staining
Listeria Monotcytogenes culture
Magnetic Columns
Magnetic Columns - CD8s
Magnetic Columns - DCs
Monomer Folding
Neuraminidase Treatment
PCR of Tail DNA
PCR Real-time
PCR Transgene Screening
Peritoneal Exudate Cells
Pretussis Toxin Treatment of Mice

Protein Gels
Purify DNA- Transfection

RNA Isolation
Supplementum Complementum
Thawing Cells
Tissue culture
Transgenic
Western Blot
Western Blot (PEP)




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