Homepage	University of Minnesota	U of M Center for Immunology

Welcome to the Hogquist/Jameson Lab!

We’re glad you are part of the group.

The lab web page is at http://www.jamequist.umn.edu

Check it out. It has current group meeting schedules, lab publications, protocols, pictures, and more! Please provide the webmaster with your biosketch and research interests (when you decide what they are!!).

Before you start working at the bench there are things you need to do to satisfy safety and security regulations at the U of M. Yes, it’s a bureaucratic zoo out there... but, we have to do it! So check it off as you get each done.

____ 1. U Card access:

a. Begin by accecessing the online training by visiting the website: http://www.dehs.umn.edu/training/#6. Once at the website, click on "Radiation Safety Orientation". The University's Internet Log-on screen will appear asking you to enter your X500 Internet ID and password.

b. When you pass the test, a message will appear to confirm this and an email will be sent to you confirming you have competed the training. (You may want to keep an extra copy of this for your own records)

c. After completing the training, bring your U-card to the BSBE front desk, so they can make a photocopy of your U-card. Also, they will ask you for your ID number (either student or employee ID numbers are on your U-card).

d. Ask Annette Bethke send an email to building management (bldg-mgmt@mail.ahc.umn.edu) containing

1) your name

2) your U card number (the set of 11 digits following 600953 on the second line of your card and

3) the hours that you will need access to the building.

After all this is done, you will still have to wait 2-3 days before you actually have U-card access to BSBE.

____2. Working with animals

a. Take the RAR orientation seminar or complete the on-line tutorial about working with laboratory animals http://www.iacuc.umn.edu/training/index.cfm NOTE: a copy of the documentation that you took this training has to be kept in the lab as well, so please file a copy of your completion certificate with the lab safety officer.

b. Call RAR to arrange one-on-one “SPF” training from. 4-6169

c. Submit a request for U-Card Access to our mouse-rooms

____3. Lab Safety: Talk to the Lab Safety Officer, read the Chemical Hygiene Plan, (note: the lab safety officer has a printed version with important sections highlighted) and sign the appropriate Lab Safety documentation. These three sheets must be filed on every person who works in the lab. (Note: You will need acrobat reader to see the following forms)

a. Hazardous Waste Generator Record of Training

b. Bloodborne Pathogen & Other Infections Agents

c. Laboratory Safety Standard Record of Training

____4. Radiation Safety: Everyone that joins the lab must receive Radiation Safety training. Go to Diehl Hall and view the “real” Radiation Safety Video. Take the test and submit results to Radiation Protection Services. File a record of training form with the Lab Safety officer.

____5. HIPPA: Take the appropriate online Health Information Protection (HIPPA) e-courses at https://www.my.ahc.umn.edu/metadot/index.pl (if you are a full-time U of M student or employee)

____6. Flow cytometry. Talk to Paul Champoux (6-3068) for a cytometer “inservice” and to become an authorized user. Watch the BD “Introduction to flow cytometry” CD if you are new to flow.

Once you start doing experiments, we ask that you keep an electronic copy of your notebook and document any protocols that you use routinely in the “Standard Operating Protocols” format. (see below)

Electronic notebook:

1. Create a lab notebook template using the following outline Lab notebook template. (Note: you can modify this outline to your liking, just be consistent).

2. Embed your analyzed data into the notebook pages whenever practical. If it is not practical, you must note clearly where (on a separate disk, in a notebook, etc.) the data is. This is critical for the PIs. We are obligated to keep primary data for 5 years after the experiment is performed. And many times, this is long after you have gone!!

3. Print your e-notebook pages out and file them in a 3-ring notebook.

4. Back-up your computer AT LEAST every 6 months.

Standard Operating Procedures:

1. Create a standard operating procedure using the following outline Standard Operating Procedure Template.

2. A new protocol is needed only if the lab web page does NOT have a current protocol for what you are doing.

3. Save it in your notebook, the SOP notebook, and on the lab web page (submit it to the webmaster for addition to the webpage).